Transcriptomics

Refining expression analysis

Refining expression analysis through more specific techniques gives a clear figure of protein involved in any biological phenomenon. The Association of Bimolecular Resource Facilities (ABRF) is dedicated to advancing core and research biotechnology laboratories through research, communication, and education. ABRF members include over 800 scientists representing 267 different core laboratories in 41 countries, including those in industry, government, academic and research institutions. RNA-Seq and Microarray Gene Expression are the two major tools used for gene expression analysis. Around 178 companies in USA are involved in the manufacturing of the equipment used for next generation sequencing and DNA microarray analysis. Mentioning some of the giants manufacturer for Transcriptome Profiling by RNA-Seq are the Applied Biosysytems and Illumina being the leader in the field. For Microarray, Affymetrix is the most reliable company, providing the quality from decades. ABRF Next-Generation Sequencing (ABRF-NGS) is the most effective technique for accurate sequencing data. For Ribonucleic acid, Single-cell RNA sequencing (scRNA-seq) is carried out which examines the RNA sequence information from individual cells with optimized next generation sequencing (NGS) technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment.