Transcriptome

The transcriptome is a collection of all RNA present in a cell or a population of cells at any given moment. The transcriptome is dynamic, as the levels of RNA transcripts vary during different developmental stages or in response to certain conditions.

Transcriptome sequencing or RNA-Seq is a next-generation sequencing (NGS)-based approach to profiling and analysing RNA. This technique delivers unbiased information without the need for prior knowledge of the genome or transcriptome. Transcriptome sequencing is often the method of choice for analysis of differentially expressed genes, as well as for RNA editing and profiling of allele-specific gene expression. RNA-seq can also be used to investigate splicing patterns, splicing variants, gene isoforms, single nucleotide polymorphisms and post transcriptional modifications.

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