Nehir Ozdemir Ozgenturk
Yildiz Technical University, Turkey
Title: Preparation of de novo transcriptome analysis from turkish filbert (Corylus colurna L.) and tombul hazelnut (Corylus avellana L. Tombul)
Biography
Biography: Nehir Ozdemir Ozgenturk
Abstract
Hazelnut has become a valuable nutrient throughout the history. As it is used in cake and chocolate products as well as appetizers, its economic value is high since oil extraction from its plant. It is quite important plant and has high economic value for Turkey that 70% of world need is supplied from Turkey. A lot of hazelnut species grow in Turkey, but the most widespread species are Corylus avellana L. Tombul which has high economically value because of their special flavor and oil content and Turkish hazelnut Corylus colurna which is used for breeding as rootstock because of their resistance against extreme nature conditions. In this study, total RNA with RNeasy Plant Mini Kit (Qiagen) was isolated from filberts (cv. Tombul) and Turkish filberts young leaves, flowers (male and female), bud, husk, shoot from the collection of Giresun Hazelnut Research Institute. From this total RNA RNA pool was composed dafter processing of cleaning with RNA MinElute Kit (Qiagen). The concentration of RNA was measured with Bio-spec-nano UV-VIS Specthrophometer. Pair-end (2x100 bp) sequencing was performed using an Illumina HiSeqTM 4000 sequencing system (Illumina) at Beijing Genomics Institute (BGI). As a pro-process, for converting of raw RNA-seq data into clean data, adaptors and low-quality reads were1 determined with FastQC and removed via FlexBar program. End this study a wide trascriptome analysis will be done for Anatolia originated two hazelnuts that most quality Tombul hazelnut (Corylus avellana L.) and used as rood stocks Turkish hazelnut (Corulus colurna L.) and this will be given wide information about their gene expression profile.