Farhid Hemmatzadeh
The University of Adelaide
Title: Transcriptomics analysis of chicken spleen cells to highly pathogenic genotype 7 of Newcastle disease virus
Biography
Biography: Farhid Hemmatzadeh
Abstract
Newcastle disease (ND) as an endemic disease in Indonesia continues to cause mortality and reduce profitability in the chicken industry. Recent investigations have shown that, despite a high level of vaccination, NDV caused major outbreaks among commercial poultry farms in Southeast Asia especially in Indonesia. Isolations of different genotypes of NDV in Indonesia have been reported by a few researchers from 2010 to 2017. It was reported that genotype-7 (G7) of highly virulent NDV caused high mortality with both gastrointestinal and neurotically signs. The mechanism of pathogenicity of newly emerged genotype 7 of NDVs and its roles in mass destruction of reticuloendothelial organs hasn’t been studied. To describe the immune response mechanisms to velogenic strains of G7-NDVs we have compares the transcriptomes of spleen tissues of six experimentally infected SPF chicken with uninfected healthy chicken. RNAseq and gene expression analysis in two infected and control groups, identified a highly significant up-regulation of host-pathogen-interactions genes including interleukin 4, GTPase, cathepsin L1-like and interferon pathway genes plus few other genes important for viral replication or the host defense. The data also demonstrate wide ranges of up regulated and down regulated gene modules that are responsible for cytokine storm and innate immune response. Our finding in this gene expression study has confirming the mass destruction of lymphocytes at the reticuloendothelial tissues in histo-pathological studies. Finally the viral load in different using qPCR assays was detected in different tissues in infected group and pre- and post-infection NDV antibodies titers were measured in in infected group as the confirmation signs for the acute infection.