Nandlal Bhojraj
Jagadguru Sri Shivarathreeshwara University, India
Title: Transcriptome analysis of oral samples
Biography
Biography: Nandlal Bhojraj
Abstract
Statement of the Problem: Analysis of transcriptome of cells present in saliva and buccal cheek provide key information about the expression of genes in healthy individuals. Knowledge about the expression profile of genes helps to determine the key players involved in keeping the oral health in good condition. However, to date, not much is known about the ideal sample, salivary cells or buccal cheek cells, to analyze for measuring the expression profile of genes. Therefore, in this study, we have compared the expression profile (at mRNA level) of cells collected from saliva of normal healthy individuals with the cells harvested from buccal cheek of the same individual.
Methodology & Theoretical Orientation: Total RNA was isolated from the cells present in saliva and buccal cheek of 15 young adults and quality assessed using TapeStation. The quality RNA was processed as shown in Image and differentially expressed genes identified. Genes were considered significantly differentially expressed, if their q values <0.05 from ANOVA tests and log2 Fold change value is above and below 1.
Findings: Quality RNA could be extracted from both saliva and buccal cheek cells, Yield of quality RNA is much better if the sample is buccal swab and among a total of 140 RNAs differentially expressed between saliva and buccal cheek cells, 15 genes found to be upregulated and 135 genes appeared to be down regulated.
Conclusion & Significance: In conclusion, results of this study demonstrate that the ideal sample for RNA isolation and subsequent transcriptome analysis is buccal cheek but not the saliva