Day 1 :
Keynote Forum
Nehir Ozdemir Ozgenturk
Yildiz Technical University
Keynote: RNA-seq based transcriptome analysis of thymus tissues of MMTV-TGF- α mice in calorie restriction fed
Time : 10:30-11:30
Biography:
Nehir Ozdemir Ozgenturk has been working at Yildiz Technical University since 2002. She has completed her Doctorate at Justus Liebieg University in Germany in 2000 by preparing the BAC library for sunflower. She is continuing her genomic researches by analyzing RNA seq and transcriptome analysis
Abstract:
Thymus is an organ that plays a critical role in the development of the immune system, while nutrition has a great influence on our immune system. Calorie Restriction (CR), an important player in prolonging the life span and in immune responses, is an effective protective method in the development of cancer. RNA-seq based transcriptome sequencing was performed to thymus tissues of MMTV-TGF- α mice in ad libitum (AL), chronic calorie restriction (CCR) and intermittent calorie restriction (ICR) fed in this study. We report the use of RNA sequencing to find the differential expressed genes (DEGs) that affected by different feeding regimes in thymus tissue. Three cDNA libraries were sequenced using Illumina HiSeq 4000 to produce 100 base pair-end reads. On average 115 million clean reads were mapped to GRCm38 mouse reference genome by TopHat2. In total 6091 significant differential expressed genes (DEGs) were identified with Cuffdiff (p<0.05). These DEGs were clustered into gene ontology (GO) categories. The expression pattern revealed by RNA-seq was validated by quantitative real-time PCR (qPCR) analysis of four important genes. RNA-seq data has been deposited in NCBI gene expression omnibus (GEO) database (GSE95371). The results suggest that RNA-seq is a useful tool to predict differences in gene expression levels between distinct situations. Moreover, results of this study will provide an important source for nutrition, cancer and immune system researches.
Keynote Forum
Farhid Hemmatzadeh
The University of Adelaide
Keynote: Transcriptomics analysis of chicken spleen cells to highly pathogenic genotype 7 of Newcastle disease virus
Time : 11:50-12:50
Biography:
Farhid Hemmatzadeh is a virologist at the School of Animal and Veterinary Sciences, University of Adelaide in Australia. He has been involved in research, development and assessment of diagnostic test for avian influenza in South East Asia especially in Indonesia. He has supervised numbers of PhD project and led international collaboration in the field of avian Influenza, Newcastle disease, Bovine Virus Diarrhea viruses and animal retroviruses
Abstract:
Newcastle disease (ND) as an endemic disease in Indonesia continues to cause mortality and reduce profitability in the chicken industry. Recent investigations have shown that, despite a high level of vaccination, NDV caused major outbreaks among commercial poultry farms in Southeast Asia especially in Indonesia. Isolations of different genotypes of NDV in Indonesia have been reported by a few researchers from 2010 to 2017. It was reported that genotype-7 (G7) of highly virulent NDV caused high mortality with both gastrointestinal and neurotically signs. The mechanism of pathogenicity of newly emerged genotype 7 of NDVs and its roles in mass destruction of reticuloendothelial organs hasn’t been studied. To describe the immune response mechanisms to velogenic strains of G7-NDVs we have compares the transcriptomes of spleen tissues of six experimentally infected SPF chicken with uninfected healthy chicken. RNAseq and gene expression analysis in two infected and control groups, identified a highly significant up-regulation of host-pathogen-interactions genes including interleukin 4, GTPase, cathepsin L1-like and interferon pathway genes plus few other genes important for viral replication or the host defense. The data also demonstrate wide ranges of up regulated and down regulated gene modules that are responsible for cytokine storm and innate immune response. Our finding in this gene expression study has confirming the mass destruction of lymphocytes at the reticuloendothelial tissues in histo-pathological studies. Finally the viral load in different using qPCR assays was detected in different tissues in infected group and pre- and post-infection NDV antibodies titers were measured in in infected group as the confirmation signs for the acute infection.